Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD19 antibody [SP110] - BSA and Azide free (ab240402)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP110] to CD19 - BSA and Azide free
Suitable for: IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-CD19 antibody [SP110] - BSA and Azide free
See all CD19 primary antibodies
Description

Rabbit monoclonal [SP110] to CD19 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cytmore details
Species reactivity

Reacts with: Human
Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil tissue Flow Cyt: Raji cells
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab240402 is the carrier-free version of ab225717. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240402 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by Protein A/G.
Clonality

Monoclonal
Clone number

SP110
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD19 antibody [SP110] - BSA and Azide free (ab240402)

Flow cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified ab225717 at 1/40 dilution (11.725 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225717).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP110] - BSA and Azide free (ab240402)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD19 using ab225717 at 1/200 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab225717).
Flow Cytometry - Anti-CD19 antibody [SP110] - BSA and Azide free (ab240402)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling CD19 with ab225717 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225717).
Anti-CD19 antibody [SP110] - BSA and Azide free (ab240402)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com