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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MARK

Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)

Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8553] to MARK2 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-MARK2 antibody [EPR8553] - BSA and Azide free
    See all MARK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8553] to MARK2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248629 is the carrier-free version of ab133724. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248629 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.70 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8553
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MARK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)

    This data was developed using ab133724, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: MARK2 knockout HAP1 cell lysate (20 µg)

    Lane 3: K562 cell lysate (20 µg)

    Lane 4: Raw264.7 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133724 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133724 was shown to recognize MARK2 when MARK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MARK2 knockout samples were subjected to SDS-PAGE. ab133724 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    Western blot - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    All lanes : Anti-MARK2 antibody [EPR8553] (ab133724) at 1/1000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : NIH/3T3 cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa



    This data was developed using ab133724, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    Flow Cytometry - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    This data was developed using ab133724, the same antibody clone in a different buffer formulation.Flow cytometry analysis of permeabilized Raji cells staining MARK2, using ab133724 at a 1/100 dilution (red) or a control rabbit IgG antibody (green).
  • OI-RD Scanning - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    OI-RD Scanning - Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    This data was developed using ab133724, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)
    Anti-MARK2 antibody [EPR8553] - BSA and Azide free (ab248629)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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