Antibodies, Proteins, Kits and Reagents for Life Science

Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [NORI138B] to p75 NGF Receptor
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-p75 NGF Receptor antibody [NORI138B]
See all p75 NGF Receptor primary antibodies
Description

Rat monoclonal [NORI138B] to p75 NGF Receptor
Host species

Rat

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is considered to be commercially sensitive.
Positive control
- WB: Human brain, Neuro-2a, Mouse brain and Rat brain, PC-12 lysates. IHC-P: Human tonsil tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

NORI138B
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling p75 NGF with ab271289 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the germinal center and basement membrane on the epithelium in the human tonsil. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0)
Western blot - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

All lanes : Anti-p75 NGF Receptor antibody [NORI138B] (ab271289) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 45 kDa
Observed band size: 65 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: lane 1, 2 : 3 minutes lane 3-5 : 59 seconds
Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com