Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [NORI138B] to p75 NGF Receptor
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p75 NGF Receptor antibody [NORI138B]
    See all p75 NGF Receptor primary antibodies

  • Description

    Rat monoclonal [NORI138B] to p75 NGF Receptor

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human brain, Neuro-2a, Mouse brain and Rat brain, PC-12 lysates. IHC-P: Human tonsil tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling p75 NGF with ab271289 at 1/1000 dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the germinal center and basement membrane on the epithelium in the human tonsil. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0)

  • Western blot - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    Western blot - Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    All lanes : Anti-p75 NGF Receptor antibody [NORI138B] (ab271289) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Rat brain tissue lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: lane 1, 2 : 3 minutes lane 3-5 : 59 seconds

  • Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)
    Anti-p75 NGF Receptor antibody [NORI138B] (ab271289)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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