Anti-GDNF antibody (ab18956)
Key features and details
- Rabbit polyclonal to GDNF
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-GDNF antibody
See all GDNF primary antibodies -
Description
Rabbit polyclonal to GDNF -
Host species
Rabbit -
Specificity
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide surrounding amino acid 195 of mouse GDNF.(Peptide available as ab51926.)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-GDNF antibody (ab18956) This image is courtesy of an abreview submitted by Tamas Bellak, University of Szeged.
Immunocytochemistry/ Immunofluorescence analysis of genetically modified rat embryonic fibroblasts labeling GDNF with ab18956 at 1/150 dilution. Cells were fixed in formaldehyde and permeabilized with Tx-100. Staining with ab18956 at 1/150 was carried out for 14 hours at 4°C in PBS buffer. A Goat Anti-Rabbit (Alexa Fluor® 594) secondary antibody was used at 1/600 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-GDNF antibody (ab18956)ICC/IF image of ab18956 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18956, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
