Anti-GDNF antibody [EPR2714(N)] (ab176564)
![Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.com/ps/products/176/ab176564/Images/ab176564-204269-anti-gdnf-antibody-epr2714n-western-blot.jpg "Anti-GDNF antibody [EPR2714(N)] (ab176564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2714(N)] to GDNF
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-GDNF antibody [EPR2714(N)]
See all GDNF primary antibodies -
Description
Rabbit monoclonal [EPR2714(N)] to GDNF -
Host species
Rabbit -
Specificity
GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
-
Immunogen
Synthetic peptide within Human GDNF aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P39905 -
Positive control
- SH-SY5Y and HepG2 whole cell lysate (ab7900), SH-SY5Y cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2714(N) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-GDNF antibody [EPR2714(N)] (ab176564)All lanes : Anti-GDNF antibody [EPR2714(N)] (ab176564) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
-
![Flow Cytometry - Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.com/ps/products/176/ab176564/Images/ab176564-204268-anti-gdnf-antibody-epr2714n-flow-cytometry.jpg)
Flow Cytometry - Anti-GDNF antibody [EPR2714(N)] (ab176564)Flow cytometry analysis of permeabilized SH-SY5Y cells labeling GDNF (red) with ab176564 at a 1/10 dilution, or negative control rabbit IgG (green)
-
![Anti-GDNF antibody [EPR2714(N)] (ab176564)](https://www.abcam.com/ps/products/176/ab176564/Images/ab176564-3-benefits-of-recombinant-antibodies.png)
Anti-GDNF antibody [EPR2714(N)] (ab176564)
