Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8304(B)] to ARF1+ARF3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free
    See all ARF1+ARF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8304(B)] to ARF1+ARF3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248253 is the carrier-free version of ab129003. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248253 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)
    Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)
    All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/20000 dilution

    Lane 1 : Full length human ARF-1 recombinant protein
    Lane 2 : Full length human ARF-3 recombinant protein

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab129003, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human ARF-1 recombinant protein (ab123538), Human ARF-3 recombinant protein (ab113581).

  • Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)
    Western blot - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)
    All lanes : Anti-ARF1 + ARF3 antibody [EPR8304(B)] (ab129003) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa



    This data was developed using ab129003, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)
    This data was developed using ab129003, the same antibody clone in a different buffer formulation.ab129003, at 1/50 dilution, staining ARF3 in paraffin-embedded Human stomach tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)
    Anti-ARF1+ARF3 antibody [EPR8304(B)] - BSA and Azide free (ab248253)

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