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Neuroscience Neurology process Neural Signal Transduction

Anti-AKT1 antibody (ab59380)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-AKT1 antibody (ab59380)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to AKT1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG

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Overview

  • Product name

    Anti-AKT1 antibody
    See all AKT1 primary antibodies
  • Description

    Rabbit polyclonal to AKT1
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total AKT1 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, African green monkey
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human AKT1 aa 292-341. Synthesized non-phosphopeptide derived from human AKT1 around the phosphorylation site of tyrosine 326.
    Database link: P31749

  • Positive control

    • WB: HeLa cells, treated with Insulin 0.01U/ml for 15 minutes; Cos-7, HepG2 cells. IHC-P: Human brain tissue. ICC: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-AKT1 antibody (ab59380)
    Western blot - Anti-AKT1 antibody (ab59380)
    All lanes : Anti-AKT1 antibody (ab59380) at 1/500 dilution

    Lane 1 : HeLa cells, treated with Insulin 0.01U/ml for 15 minutes
    Lane 2 : synthesized peptide

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody (ab59380)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody (ab59380)

    Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ab59380 at 1/50 dilution. The picture on the right is blocked with the synthesized peptide

  • Immunocytochemistry/ Immunofluorescence - Anti-AKT1 antibody (ab59380)
    Immunocytochemistry/ Immunofluorescence - Anti-AKT1 antibody (ab59380)

    Immunofluorescence analysis of HeLa cells, using ab59380 at 1/100 dilution. The picture on the right is blocked with the synthesized peptide.

  • Western blot - Anti-AKT1 antibody (ab59380)
    Western blot - Anti-AKT1 antibody (ab59380)
    Anti-AKT1 antibody (ab59380) at 1/1000 dilution + Cos-7 cells

    Predicted band size: 56 kDa

  • Western blot - Anti-AKT1 antibody (ab59380)
    Western blot - Anti-AKT1 antibody (ab59380)
    Anti-AKT1 antibody (ab59380) at 1/1000 dilution + HepG2 cells

    Predicted band size: 56 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AKT1 antibody (ab59380)

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