Anti-ACF1 / BAZ1A antibody [EPR15826] - BSA and Azide free (ab250942)
![Anti-ACF1 / BAZ1A antibody [EPR15826] - BSA and Azide free (ab250942)](https://www.abcam.com/ps/products/250/ab250942/Images/ab250942-389780-anti-acf1-baz1a-antibody-epr15826-c-terminal-western-blot-human-lysate.jpg "Anti-ACF1 / BAZ1A antibody [EPR15826] - BSA and Azide free (ab250942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15826] to ACF1 / BAZ1A - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ACF1 / BAZ1A antibody [EPR15826] - BSA and Azide free
See all ACF1 / BAZ1A primary antibodies -
Description
Rabbit monoclonal [EPR15826] to ACF1 / BAZ1A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat and Daudi cell lysates.
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General notes
Ab250942 is the carrier-free version of ab187670. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250942 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15826 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - BSA and Azide free (ab250942)All lanes : Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (ab187670) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BAZ1A knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 178 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab187670).
Lanes 1-4: Merged signal (red and green). Green - ab187670 observed at 178 kDa. Red - loading control ab7291 observed at 50 kDa.
ab187670 Anti-BAZ1A antibody [EPR15826] - C-terminal was shown to specifically react with BAZ1A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264925 (knockout cell lysate ab257856) was used. Wild-type and BAZ1A knockout samples were subjected to SDS-PAGE. ab187670 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-ACF1 / BAZ1A antibody [EPR15826] - BSA and Azide free (ab250942)](https://www.abcam.com/ps/products/250/ab250942/Images/ab250942-2-benefits-of-recombinant-antibodies.png)
Anti-ACF1 / BAZ1A antibody [EPR15826] - BSA and Azide free (ab250942)
