Anti-Acetylcholinesterase antibody (ab97299)
Key features and details
- Rabbit polyclonal to Acetylcholinesterase
- Suitable for: IHC-Fr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Acetylcholinesterase antibody
See all Acetylcholinesterase primary antibodies -
Description
Rabbit polyclonal to Acetylcholinesterase -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat, Monkey
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Immunogen
Recombinant fragment corresponding to Human Acetylcholinesterase aa 406-610.
Database link: P22303 -
Positive control
- ICC/IF: HeLa IHC-Fro: E13.5 rat brain. IHC-P: Rat fore brain and brain, mouse colon and brain. WB: Raji cells, mouse and rat brain. Mouse lung.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (ab97299)
Paraffin embedded mouse brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-Acetylcholinesterase antibody (ab97299)All lanes : Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution
Lane 1 : Mouse brain
Lane 2 : Mouse lung
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (ab97299)Paraffin embedded mouse colon tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-Acetylcholinesterase antibody (ab97299)Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution + Rat brain at 50 µg
Predicted band size: 68 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (ab97299)Paraffin embedded rat brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody (ab97299)Paraffin embedded rat fore brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-Acetylcholinesterase antibody (ab97299)All lanes : Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution
Lane 1 : Mouse Brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa
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Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody (ab97299)Frozen sectioned E13.5 rat brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-Acetylcholinesterase antibody (ab97299)Anti-Acetylcholinesterase antibody (ab97299) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Acetylcholinesterase antibody (ab97299)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97299 (1) and merged with DNA probe (2) at 1/200 dilution.
