Anti-Acetylcholinesterase antibody (ab31276)
Key features and details
- Goat polyclonal to Acetylcholinesterase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Acetylcholinesterase antibody
See all Acetylcholinesterase primary antibodies -
Description
Goat polyclonal to Acetylcholinesterase -
Host species
Goat -
Specificity
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
QFDHYSKQDRCSDL
, corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase -
Positive control
- Human fibroblast cell lines A431 and HeLa lysates. Human cerebellum lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Acetylcholinesterase antibody (ab31276) This image is courtesy of an Abreview submitted by Ender AvciAll lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution
Lane 1 : SkHep1 cell lysate
Lane 2 : Snu387 cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : Zebrafish brain lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 20 minutes
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Western blot - Anti-Acetylcholinesterase antibody (ab31276)Anti-Acetylcholinesterase antibody (ab31276) at 0.1 µg/ml + Human Fibroblast A431 lysate (35µg protein in RIPA buffer)
Predicted band size: 68 kDa
Observed band size: 60-70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-Acetylcholinesterase antibody (ab31276)Anti-Acetylcholinesterase antibody (ab31276) at 1 µg/ml + human cerebellum lysate (35µg protein in RIPA buffer).
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.Detected by chemiluminescence.
