Anti-PD-L1 antibody [CAL10] (ab279292)
![Anti-PD-L1 antibody [CAL10] (ab279292)](https://www.abcam.com/ps/products/279/ab279292/Images/ab279292-450236-anti-pdl1-antibody-cal10msigg1-western-blot-chos.jpg "Anti-PD-L1 antibody [CAL10] (ab279292)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [CAL10] to PD-L1
- Suitable for: WB, ICC, Flow Cyt (Intra), IHC-P
- Reacts with: Human, Chinese hamster
Overview
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Product name
Anti-PD-L1 antibody [CAL10]
See all PD-L1 primary antibodies -
Description
Mouse monoclonal [CAL10] to PD-L1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt (Intra) Chinese hamsterICC Chinese hamsterIHC-P HumanWB HumanChinese hamster
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PD-L1 stably expressed CHO whole cell lysate. Human placenta tissue lysate. NCI-H1299 whole cell lysate. ICC: PD-L1 stably expressed CHO cells. Flow Cyt: PD-L1 stably expressed CHO cells. IHC-P: Human tonsil tissue.
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General notes
This mouse antibody has been engineered from a RabMAb parent antibody (ab237726). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CAL10 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PD-L1 antibody [CAL10] (ab279292)All lanes : Anti-PD-L1 antibody [CAL10] (ab279292) at 1/1000 dilution
Lane 1 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate
Lane 2 : CHO-PD-L1 (PD-L1 stably expressed Chinese hamster ovary epithelial cell) whole cell lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Observed band size: 40-60 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [CAL10] (ab279292)](https://www.abcam.com/ps/products/279/ab279292/Images/ab279292-450191-anti-pdl1-antibody-CAL10-immunohistohemistry-tonsil-human-YR061520PS-MsIgG1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [CAL10] (ab279292)IHC image of PD-L1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab279292, 1ug/ml, for 15 mins at room temperature. A rabbit anti-mouse IgG1, ab125913, was added for 8 mins at room temperature and detected using an HRP conjugated goat anti-rabbit compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunocytochemistry - Anti-PD-L1 antibody (ab279292)Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100-fixed permeabilized CHO-PD-L1 cells labeling PD-L1 with ab279292 at 1/100 dilution, followed by ab150113 Goat Anti-MouseIgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue). Confocal image showing membranous and cytoplasmic staining in CHO-PD-L1 cells.
Negative control 1: ab279292 at a 1/100 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
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Flow Cytometry (Intracellular) - Anti-PD-L1 antibody (ab279292)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized CHO-s (Chinese hamster ovary epithelial cell, Blue) / CHO-PDL1 (PD-L1 stably expessed Chinese hamster ovary epithelial cell, Red) labelling PD-L1 with ab279292 at 1/50 dilution (0.1µg).
Goat Anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.