Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calreticulin antibody - ER Marker (ab39897)

Key features and details

  • Rabbit polyclonal to Calreticulin - ER Marker
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Calreticulin antibody - ER Marker
    See all Calreticulin primary antibodies

  • Description

    Rabbit polyclonal to Calreticulin - ER Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Calreticulin aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab39896)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line); Jurkat (Human T cell lymphoblast-like cell line); HepG2 (Human hepatocellular liver carcinoma cell line); A431 (Human epithelial carcinoma cell line).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab39897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
All applications
Rat
Rabbit
Chinese hamster
Application Abreviews Notes
WB
1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
Notes
WB
1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.

  • Sequence similarities

    Belongs to the calreticulin family.

  • Domain

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.

  • Cellular localization

    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Images

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Calreticulin knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: NIH3T3 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab39897 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab39897 was shown to recognize Calreticulin when Calreticulin knockout samples were used, along with additional cross-reactive bands. Wild-type and Calreticulin knockout samples were subjected to SDS-PAGE. ab39897 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)
    All lanes : Anti-Calreticulin antibody - ER Marker (ab39897) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : Hep G2 whole cell lysate (ab7900)
    Lane 4 : A-431 whole cell lysate (ab7909)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.



    The predicted band size is 48kDa based on Swiss-prot data, however a band of 55kDa is consistent with that observed in other commercially available antibodies to Calreticulin. This difference in size is likely to be the result of post-translational modification.

References (1)

Publishing research using ab39897? Please let us know so that we can cite the reference in this datasheet.

ab39897 has been referenced in 1 publication.

  • Vannucchi AM  et al. Calreticulin mutation-specific immunostaining in myeloproliferative neoplasms: pathogenetic insight and diagnostic value. Leukemia N/A:N/A (2014). Human . PubMed: 24618731

Images

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Calreticulin knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: NIH3T3 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab39897 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab39897 was shown to recognize Calreticulin when Calreticulin knockout samples were used, along with additional cross-reactive bands. Wild-type and Calreticulin knockout samples were subjected to SDS-PAGE. ab39897 and ab8245 (loading control to GAPDH) were diluted 1/250 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab39897)
    All lanes : Anti-Calreticulin antibody - ER Marker (ab39897) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : Hep G2 whole cell lysate (ab7900)
    Lane 4 : A-431 whole cell lysate (ab7909)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.



    The predicted band size is 48kDa based on Swiss-prot data, however a band of 55kDa is consistent with that observed in other commercially available antibodies to Calreticulin. This difference in size is likely to be the result of post-translational modification.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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