Human MAPK Phosphorylation Antibody Array (Membrane, 17 Targets) (ab211061)
Overview
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Product name
Human MAPK Phosphorylation Antibody Array (Membrane, 17 Targets) -
Sample type
Cell Lysate, Tissue Lysate -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human MAPK Phosphorylation Antibody Array (ab211061) for use with cell and tissue lysates.
Targets: Akt (pS473), CREB (pS133), ERK1 (pT202/Y204)/ERK2 (pT185/Y187), GSK3a (pS21), GSK3b (pS9), HSP27 (pS82), JNK (pT183), MEK (pS217/221), MKK3 (pS189), MKK6 (pS207), MSK2 (pS360), mTOR (pS2448), p38 (pT180/Y182), p53 (pS15), P70S6K (pT421/S424), RSK1 (pS380), RSK2 (pS386).
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Notes
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each membrane), and then paired detector antibodies and HRP-Anti-Rabbit IgG. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about cytokine arrays and other membrane antibody arrays
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 2 Membrane 4 Membrane 8 Membrane 1,000X HRP-Anti-Rabbit IgG 1 x 20µl 1 x 20µl 1 x 20µl 100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 1 vial 2 vials 20X Wash Buffer I 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Antibody Arrays 2 units 4 units 8 units Detection Antibody Cocktail 1 vial 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Phosphatase Inhibitor Cocktail Set II 1 vial 1 vial 2 vials Plastic sheets 1 unit 1 unit 1 unit Protease Inhibitor Cocktail 1 vial 1 vial 2 vials
Images
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Typical data
HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
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Typical dataHeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated or treated with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
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Typical dataHepG2 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
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Typical dataHepG2 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated or treated with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.