Recombinant human DNase I protein (Active) (ab73430)
Key features and details
- Expression system: CHO cells
- Active: Yes
- Suitable for: WB, Functional Studies
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Product name
Recombinant human DNase I protein (Active)
See all DNase I proteins and peptides -
Biological activity
1000IU/1mg.
One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH-5. (Also, 0.005 Kunitz unit digests 1µg of lambda phage DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, pH 7.8 in a 50ul reaction volume.)
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Expression system
CHO cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
37 kDa
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Preparation and Storage
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Alternative names
- Deoxyribonuclease 1
- Deoxyribonuclease I
- Deoxyribonuclease-1
see all -
Function
Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. -
Tissue specificity
Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva. -
Sequence similarities
Belongs to the DNase I family. -
Cellular localization
Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope. - Information by UniProt
Images
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Western blot - Recombinant human DNase I protein (ab73430)Anti-DNase I antibody (ab97393) at 1/1000 dilution +
Recombinant human DNase I protein (Active) (ab73430) at 0.001 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Exposure time: 4 minutes