Recombinant human IL-15RA protein (Fc Chimera Active) (ab261848)
Key features and details
- Expression system: HEK 293 cells
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Alternative names
- AA690181
- CD215
- I15RA_HUMAN
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Function
Receptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK. -
Tissue specificity
Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures. -
Sequence similarities
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
Phosphorylated by activated SYK.
N-glycosylated and O-glycosylated. -
Cellular localization
Secreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant human IL-15RA protein (Fc Chimera Active) (ab261848)
SDS PAGE analysis of ab261848 under reducing conditions.
The protein has a calculated MW of 44.8 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Functional Studies - Recombinant human IL-15RA protein (Fc Chimera Active) (ab261848)Immobilized ab261848 at 2 μg/ml (100 μl/well) can bind Biotinylated Human IL-15, Fc Tag, Avi Tag with a linear range of 2-78 ng/ml.
