Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18978] to Acetylcholinesterase
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Acetylcholinesterase antibody [EPR18978]
    See all Acetylcholinesterase primary antibodies

  • Description

    Rabbit monoclonal [EPR18978] to Acetylcholinesterase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and striatum lysates; rat striatum, hippocampus and brain lysates. IHC-P: Mouse striatum and skeletal muscle tissues; rat striatum and skeletal muscle tissues. IHC-Fr: Mouse brain (Coronal section) and rat brain (sagittal section) tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18978

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse striatum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 30 seconds, Lane 2: 3 minutes.

    Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).

  • Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain (Coronal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Mouse striatum. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution

  • Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Immunohistochemistry (Frozen sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat brain (sagittal section) tissue labeling Acetylcholinesterase with ab183591 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed high expression on Rat striatum. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

    Immunohistochemical analysis of paraffin-embedded Rat striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Western blot - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    All lanes : Anti-Acetylcholinesterase antibody [EPR18978] (ab183591) at 1/1000 dilution

    Lane 1 : Rat striatum lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Acetylcholinesterase hydrolyzes the acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission (PMID: 2400605; PMID 8515842).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

    Immunohistochemical analysis of paraffin-embedded Mouse striatum tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Mouse striatum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Mouse skeletal muscle is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

    Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Acetylcholinesterase with ab183591 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neuromuscular junction of Rat skeletal muscle is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)
    Anti-Acetylcholinesterase antibody [EPR18978] (ab183591)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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