Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)
![Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)](https://www.abcam.com/ps/products/10/ab10805/Images/ab10805-18468-anti-5-methylcytosine-5-mc-antibody-33d3-immunohistochemistry.jpg "Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)")
Key features and details
- Mouse monoclonal [33D3] to 5-methylcytosine (5-mC)
- Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-5-methylcytosine (5-mC) antibody [33D3]
See all 5-methylcytosine (5-mC) primary antibodies -
Description
Mouse monoclonal [33D3] to 5-methylcytosine (5-mC) -
Host species
Mouse -
Specificity
Modified base 5-methylcytidine found in DNA of plants and vertebrates.
Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.
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Tested applications
Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Frmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC).
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General notes
Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 0.8% Sodium chloride, 0.02% Potassium chloride, 0.03% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue. -
Clonality
Monoclonal -
Clone number
33D3 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig
ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.
