Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab73938)
Key features and details
- Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC)
- Suitable for: Southern Blot, ICC/IF
- Reacts with: Species independent
- Isotype: IgM
Overview
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Product name
Anti-5-methylcytosine (5-mC) antibody [5MC-CD]
See all 5-methylcytosine (5-mC) primary antibodies -
Description
Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) -
Host species
Mouse -
Specificity
ab73938 reacts with DNA with 5-Methylcytosine (methylated DNA). -
Tested applications
Suitable for: Southern Blot, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).
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Positive control
- Chlamidomonas me-1 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purification notes
ab73938 is purified and filter-sterilised. -
Clonality
Monoclonal -
Clone number
5MC-CD -
Isotype
IgM -
Research areas
Images
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Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamidomonas me-1 cells. Left: DAPI-stained cells. Middle: Cells stained with ab73938 and FITC-conjugated 2nd antibody. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells
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ab73938 staining 5-Methyl Cytosine in murine PN5 zygotes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol/acetic acid (3:1) and blocked with 30% serum for overnight a 4°C. Samples were incubated with primary antibody (1/100 in diluent) for 18 hours at 4°C before detection with a fluorescent secondary antibody.