Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) -
Biological activity
Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells. -
Purity
>= 95 % SDS-PAGE.
ab108556 is 0.2 µm filtered. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Human -
Predicted molecular weight
50 kDa including tags -
Additional sequence information
Human FGF21 (aa 1-209) is fused at the C-terminus to the Fc portion of human IgG1.
Description
Recombinant human FGF21 + IgG1 protein (Fc Chimera Active)Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab108556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Working aliquots are stable for up to 3 months when stored at -20°C. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Cellular localization
FGF21: Secreted. IgG1: Secreted. Cell membrane; Single-pass membrane protein
Images
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Functional Studies - Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF 21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: 1 μg/ml hFGF21-His (competitor)
Lane 2: 2.2 μg/ml ab108556 (hFGF21-Fc)
Lane 3: 2.2 μg/ml mCD137-Fc
Lane 4: 100 ng/μl FGF-b
Lane 5: control (non-treated)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab108556 has not yet been referenced specifically in any publications.
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF 21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: 1 μg/ml hFGF21-His (competitor)
Lane 2: 2.2 μg/ml ab108556 (hFGF21-Fc)
Lane 3: 2.2 μg/ml mCD137-Fc
Lane 4: 100 ng/μl FGF-b
Lane 5: control (non-treated)