Adenylate Kinase Assay Kit (Cytotoxicity) (ab228557)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Luminescent
- Platform: Microplate reader
- Sample type: Cell culture media
Overview
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Product name
Adenylate Kinase Assay Kit (Cytotoxicity) -
Detection method
Luminescent -
Sample type
Cell culture media -
Assay type
Enzyme activity (quantitative) -
Product overview
Adenylate Kinase Assay Kit (Cytotoxicity) (ab228557) measures the activity of the adenylate kinase released by damaged cells in a simple one-step procedure involving two chemical reactions.
In the first reaction, adenylate kinase converts ADP to ATP. In the second reaction, luciferase catalyzes the formation of light from ATP and luciferin; the light is then measured using a luminometer or beta counter.
The assay is highly sensitive and can be fully automated for high throughput applications.
Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.
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Notes
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and is rapidly released into culture medium upon damage to the plasma membrane.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests AK Assay Buffer 1 x 50ml AK Detection Reagent Lyophilized 5 vials -
Research areas
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Alternative names
- adk
