Acid Phosphatase Assay Kit (Cytotoxicity) (ab273300)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Acid Phosphatase Assay Kit (Cytotoxicity)
See all Acid phosphatase kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
In the Acid Phosphatase Assay Kit (ab273300), intracellular ACP cleaves the colorless chromogenic substrate para-nitrophenyl phosphate producing a yellow colored product (p-nitrophenol) under alkaline conditions. The released chromophore can be measured at OD 405 nm and is directly proportional to the viable cell number.
This assay kit is simple, accurate, reproducible and sensitive with a dynamic range between 103 - 105 cells. It offers a simple, yet excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 500 tests 20 mM Doxorubicin 1 x 100µl ACP Buffer 1 x 50ml Stop Solution 1 x 10ml Substrate (20 tabs)(Lyophilized) 2 vials -
Research areas
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Relevance
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease. -
Cellular localization
ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein. -
Alternative names
- Acid phosphatase 1 soluble
- Acid phosphatase 2 lysosomal
- Acid phosphatase 5 tartrate resistant
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Images
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Example data
Dose-response curve of MCF-7 cells exposed to doxorubicin for 72 hr determined by the ACP cytotoxicity assay. Assays were performed according to the kit protocol in triplicate. The determined IC50 is 0.82 µM.