Recombinant rat PDE4D protein (ab198442)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant rat PDE4D protein
See all PDE4D proteins and peptides -
Biological activity
Specific Activity: ≥ 22,535 pmole/min/µg.
Assay Description: One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.
Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of rPDE4D at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Purity
>= 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDPLVPRGSPGEFEAEGSSVPARAGSDEGSDS SGGAALKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQLQ PPPPPPLPPPPPPPGATRGRYASSGASRVRHRGYSDTERYLYCRAMDRTS YAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSG LILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTP FAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQK LASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRS GNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSS LTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMH TIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTH VLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMY NDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMAIDIVLATDM SKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPT KPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFI DYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQED GRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSES TEIPLDEQVEEEAVAEEESQPQTGVADDCCPDT -
Predicted molecular weight
117 kDa including tags -
Amino acids
2 to 803 -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_001113328.
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Preparation and Storage
- cAMP specific 3',5' cyclic phosphodiesterase 4D
- DKFZp686M11213
- 5''-cyclic phosphodiesterase 4D
Images
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Functional Studies - Recombinant rat PDE4D protein (ab198442)
Activity assay of ab198442.
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SDS-PAGE - Recombinant rat PDE4D protein (ab198442)4-20% SDS-PAGE analysis of 2 µg ab198442 with Coomassie staining.
