cAMP Assay Kit (Competitive ELISA) (ab133051)
Key features and details
- Sample type: Cell culture media, Cell Lysate, Saliva, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mammals
Overview
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Product name
cAMP Assay Kit (Competitive ELISA)
See all cAMP kits -
Detection method
Colorimetric -
Sample type
Saliva, Serum, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Competitive -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
cAMP ELISA Kit ab133051 uses a sensitive colorimetric competitive ELISA method to quantify cAMP (cyclic AMP) levels in samples from mammals and other species. It has a maximum sensitivity of 0.039 pmol/mL / 0.039 nM. It is not suitable for use with samples from rabbits.
The cAMP ELISA uses a 96-well plate supplied coated with a goat anti-rabbit IgG antibody. The anti-rabbit antibody binds an anti-cAMP antibody which is added to the plate.
Alkaline phosphatase (AP)-labeled cAMP, and free cAMP within a sample, are then added to each well of the plate. The cAMP ELISA is based on the competition between the AP-labeled cAMP and the free cAMP for the fixed number of cAMP antibody binding sites on the plate.
The more free cAMP is present, the less AP-labeled cAMP is bound. After a washing step, the amount of bound AP-labeled cAMP is detected using the colorimetric AP substrate pNpp measured by absorbance at OD 405 nm. The level of signal is compared to a standard curve for free cAMP, which is run at the same time as the samples.
This kit uses an optional acetylation step to achieve the maximum sensitivity of 0.039 nM, without the acetylation step the sensitivity is 0.3 pmol/mL / 0.3 nM.
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Notes
This kit was previously called Cyclic AMP Complete ELISA Kit.
If you are using fluorometric detection, we recommend cAMP Assay Kit (Fluorometric) (ab138880).
Cross Reactivity
Compound Cross Reactivity cAMP 100% AMP ATP cGMP GMP GTP cUMP CTP -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 0.1M HCl 1 x 27ml 20X Wash Buffer Concentrate 1 x 27ml Acetylation kit - Acetic Anhydride 1 x 1ml Acetylation kit - Triethylamine 1 x 2ml Assay Buffer 2 1 x 27ml Cyclic AMP Alkaline Phosphatase Conjugate 1 x 5ml Cyclic AMP Complete Antibody 1 x 5ml Cyclic AMP Complete Standard 1 x 500µl Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Neutralizing Reagent 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
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Relevance
dblinks not applicable -
Alternative names
- 3'-5'-cyclic adenosine monophosphate
- Cyclic adenosine monophosphate
- cyclic AMP
Images
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Typical Standard Curve - Non-Acetylated Format
Representative Standard Curve using ab133051. Non-Acetylated Format.
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Typical Standard Curve - Acetylated FormatRepresentative Standard Curve using ab133051. Acetylated Format.
