cAMP ELISA Kit ab133051 uses a sensitive colorimetric competitive ELISA method to quantify cAMP (cyclic AMP) levels in samples from mammals and other species. It has a maximum sensitivity of 0.039 pmol/mL / 0.039 nM. It is not suitable for use with samples from rabbits.

The cAMP ELISA uses a 96-well plate supplied coated with a goat anti-rabbit IgG antibody. The anti-rabbit antibody binds an anti-cAMP antibody which is added to the plate.

Alkaline phosphatase (AP)-labeled cAMP, and free cAMP within a sample, are then added to each well of the plate. The cAMP ELISA is based on the competition between the AP-labeled cAMP and the free cAMP for the fixed number of cAMP antibody binding sites on the plate.

The more free cAMP is present, the less AP-labeled cAMP is bound. After a washing step, the amount of bound AP-labeled cAMP is detected using the colorimetric AP substrate pNpp measured by absorbance at OD 405 nm. The level of signal is compared to a standard curve for free cAMP, which is run at the same time as the samples.

This kit uses an optional acetylation step to achieve the maximum sensitivity of 0.039 nM, without the acetylation step the sensitivity is 0.3 pmol/mL / 0.3 nM.

• Signal Transduction
• Second Messenger
• Nucleotide Messenger
• cAMP

• Kits/ Lysates/ Other
• Kits
• ELISA Kits
• ELISA Kits
• Nucleotide Messenger ELISA kits