Cyclic AMP Direct ELISA Kit (ab133038)
Key features and details
- Sample type: Cell Lysate, Tissue
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: a wide range of other species
Overview
-
Product name
Cyclic AMP Direct ELISA Kit
See all cAMP kits -
Detection method
Colorimetric -
Sample type
Tissue, Cell Lysate -
Assay type
Competitive -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: a wide range of other species -
Product overview
Abcam’s Cyclic AMP in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cAMP antigen and a polyclonal rabbit antibody specific to cAMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cAMP captured in the plate.
-
Notes
Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.
Cross Reactivity
Compound % Cross Reactivity cAMP 100 AMP 0.33 ATP 0.12 cGMP GMP GTP cUMP CTP -
Platform
Microplate
Properties
-
Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 0.1M HCl 1 x 27ml 20X Wash Buffer Concentrate 1 x 27ml Acetylation kit - Acetic Anhydride 1 x 1ml Acetylation kit - Triethylamine 1 x 2ml cAMP Antibody 1 x 5ml Cyclic AMP Alkaline Phosphatase Conjugate 1 x 5ml Cyclic AMP Complete Standard 1 x 0.5ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Neutralizing Reagent 1 x 5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
-
Relevance
dblinks not applicable -
Alternative names
- 3'-5'-cyclic adenosine monophosphate
- Cyclic adenosine monophosphate
- cyclic AMP
Images
-
Typical Standard Curve
Representative Standard Curve using ab133038
-
Typical Standard CurveRepresentative Standard Curve using ab133038
