Recombinant human JAK1 protein (Active) (ab216194)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 35% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human JAK1 protein (Active)
See all JAK1 proteins and peptides -
Biological activity
Specific Activity: 1.0 pmol/min/μg.
Assay Conditions: Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35 and 2 μM substrate (Tyr6) using Z-Lyte assay conditions. -
Purity
>= 35 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESG GNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLK EYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEH QVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVW SFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLP CPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK -
Predicted molecular weight
59 kDa including tags -
Amino acids
866 to 1154 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_002227.
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Preparation and Storage
- JAK 1
- JAK 1A
- JAK 1B
Images
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Functional Studies - Recombinant human JAK1 protein (Active) (ab216194)
Example of specific activity of ab216194.
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SDS-PAGE - Recombinant human JAK1 protein (Active) (ab216194)10% SDS-PAGE with Coomassie staining:
Lane 1: ab216194
Lane 2: MW marker