Recombinant human PDE4D protein (ab198456)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human PDE4D protein
See all PDE4D proteins and peptides -
Biological activity
Specific Activity: ≥ 13,600 pmol/min/µg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198456 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
> 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKE KEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNK WGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLED HYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHD VDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQN LTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLD NYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERG MEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLED NREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSG SQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVID DRSPDT -
Predicted molecular weight
84 kDa including tags -
Amino acids
2 to 507 -
Tags
GST tag N-Terminus -
Additional sequence information
Immunogen is from Isoform 2 with SwissProt ID Q08499-5. Known as PDE4D2.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human PDE4D protein (ab198456)
Specific activity of ab198456.
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SDS-PAGE - Recombinant human PDE4D protein (ab198456)4-20% SDS-PAGE analysis of ab198456 with Coomassie staining.
Lane 1: 2.85 µg ab198456
Lane 2: Protein marker
