Zaprinast, cGMP phosphodiesterase inhibitor (ab120940)
Key features and details
- Specific cGMP phosphodiesterase inhibitor
- CAS Number: 37762-06-4
- Purity: > 98%
- Soluble in DMSO to 25 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Zaprinast, cGMP phosphodiesterase inhibitor -
Description
Specific cGMP phosphodiesterase inhibitor -
Biological description
Specific cGMP phosphodiesterase inhibitor, selective for PDE6, 5, 11 and 9 (IC50 values are 0.15 (PDE6), 0.76 (PDE5), 12 (PDE11) and 29 μM (PDE9)). Reputed GPR35 agonist (EC50 values are 16 and 840 nM at rat and human GPR35 receptors, respectively). Important mediator for a variety of cellular processes including vasodilation. -
Purity
> 98% -
CAS Number
37762-06-4 -
Chemical structure
Properties
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Chemical name
3,6-Dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo[4,5-d]pyrimidin-7-one -
Molecular weight
271.28 -
Molecular formula
C13H13N5O2 -
Storage instructions
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CCCOc1ccccc1c1[nH]c(=O)c2nn[nH]c2n1 -
Source
Synthetic
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Research areas
Images
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Functional Studies - Zaprinast, cGMP phosphodiesterase inhibitor (ab120940)ab76165 staining ERK1 + ERK2 (phospho T202 + Y204) in U87MG cells treated with zaprinast (ab120940), by ICC/IF. Increase of ERK1 + ERK2 (phospho T202 + Y204) expression correlates with increased concentration of zaprinast, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120940 (zaprinast) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76165 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
