Anti-Human IgA antibody [EPSISR10] (ab133660)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR10] to Human IgA
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Human IgA antibody [EPSISR10]
See all Human IgA primary antibodies -
Description
Rabbit monoclonal [EPSISR10] to Human IgA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Human IgA aa 250-350. The exact sequence is proprietary.
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Positive control
- Human plasma and tonsil lysates; Human tonsil and stomach tissues; purified Human IgA.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPSISR10 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Human IgA antibody [EPSISR10] (ab133660) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
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Anti-Human IgA antibody [EPSISR10] (ab133660) at 1/1000 dilution + Purified Human IgA at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] (ab133660)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Human IgA with ab133660 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] (ab133660)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Human IgA with ab133660 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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