Recombinant human TET1 protein (ab195354)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human TET1 protein
See all TET1 proteins and peptides -
Biological activity
Can be used for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Activity of recombinant TET1 protein was measured with a 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric). In this assay, a TET substrate is converted to hydroxymethylated products, which is recognized with a specific antibody. The ratio or amount of hydroxymethylated products is proportional to enzyme activity and can then be fluorometrically measured at 530ex/590em. The activity of the TET enzyme is in turn proportional to the relative fluorescent units measured.
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Purity
> 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ELP TCSCLDRVIQ KDKGPYYTHL GAGPSVAAVR EIMENRYGQK GNAIRIEIVV YTGKEGKSSH GCPIAKWVLR RSSDEEKVLC LVRQRTGHHC PTAVMVVLIM VWDGIPLPMA DRLYTELTEN LKSYNGHPTD RRCTLNENRT CTCQGIDPET CGASFSFGCS WSMYFNGCKF GRSPSPRRFR IDPSSPLHEK NLEDNLQSLA TRLAPIYKQY APVAYQNQVE YENVARECRL GSKEGRPFSG VTACLDFCAH PHRDIHNMNN GSTVVCTLTR EDNRSLGVIP QDEQLHVLPL YKLSDTDEFG SKEGMEAKIK SGAIEVLAPR RKKRTCFTQP VPRSGKKRAA MMTEVLAHKI RAVEKKPIPR IKRKNNSTTT NNSKPSSLPT LGSNTETVQP EVKSETEPHF ILKSSDNTKT YSLMPSAPHP VKEASPGFSW SPKTASATPA PLKNDATASC GFSERSSTPH CTMPSGRLSG ANAAAADGPG ISQLGEVAPL PTLSAPVMEP LINSEPSTGV TEPLTPHQPN HQPSFLTSPQ DLASSPMEED EQHSEADEPP SDEPLSDDPL SPAEEKLPHI DEYWSDSEHI FLDANIGGVA IAPAHGSVLI ECARRELHAT TPVEHPNRNH PTRLSLVFYQ HKNLNKPQHG FELNKIKFEA KEAKNKKMKA SEQKDQAANE GPEQSSEVNE LNQIPSHKAL TLTHDNVVTV SPYALTHVAG PYNHWV -
Predicted molecular weight
80 kDa including tags -
Amino acids
1418 to 2136 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human TET1 protein (ab195354)
Example of biological activity of ab195354.
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SDS-PAGE - Recombinant human TET1 protein (ab195354)4-20% SDS-PAGE Coomassie staining; ab195354 used at 1.8 µg. MW ~80 kDa.
