Recombinant human RANKL protein (Active) (ab256098)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at room temperature. Store at -20°C.
Constituent: 0.16% Sodium phosphate
0.2 micron filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in sterile water at 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Images
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Functional Studies - Recombinant human RANKL protein (Active) (ab256098)
Functional analysis of ab256098.
Activity of RAW-BlueTM cells. ED50 ≤ 50 ng/mL (≥ 2.0 x 104 units/mg).
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SDS-PAGE - Recombinant human RANKL protein (Active) (ab256098)SDS-PAGE analysis of ab256098 (1 μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions.
4-20% Tris-Glycine gel. Coomassie Blue staining.
