Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (ab273462)
![Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (ab273462)](https://www.abcam.com/ps/products/273/ab273462/Images/ab273462-451732-anti-acriia4-antibody-7c53a8-western-blot-hek293t.jpg "Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (ab273462)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [7C5-3A8] to Anti-CRISPR protein AcrIIA4 - BSA and Azide free
- Suitable for: WB
Overview
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Product name
Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free
See all Anti-CRISPR protein AcrIIA4 primary antibodies -
Description
Mouse monoclonal [7C5-3A8] to Anti-CRISPR protein AcrIIA4 - BSA and Azide free -
Host species
Mouse -
Specificity
Species reactivity: Bacteriophage sp.
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Tested applications
Suitable for: WBmore details -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with acrIIA4 expression vector containing a myc-His-tag®, whole cell lysate.
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General notes
ab273462 is the carrier-free version of ab273439. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab273462 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7C5-3A8 -
Isotype
IgG1
Images
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Western blot - Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (ab273462)All lanes : Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] (ab273439) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with acrIIA4 expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Exposure time: 37 secondsThis data was produced using ab273439, the same clone in a different formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (ab273462)](https://www.abcam.com/ps/products/273/ab273462/Images/ab273462-456290-benefitsofrecombinantantibodies.jpg)
Anti-Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (ab273462)
