Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)
![Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)](https://www.abcam.com/ps/products/275/ab275935/Images/ab275935-2-anti-thyroid-peroxidasetpo-antibody-epr23574130-immunohistochemistry-frozen-rat-thyroid.jpg "Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23574-130] to Thyroid Peroxidase/TPO - BSA and Azide free
- Suitable for: IHC-Fr
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free
See all Thyroid Peroxidase/TPO primary antibodies -
Description
Rabbit monoclonal [EPR23574-130] to Thyroid Peroxidase/TPO - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-Fr: Mouse thyroid tissue; Rat thyroid tissue.
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General notes
ab275935 is the carrier-free version of ab275932. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275935 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23574-130 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)
This data was developed using ab275932, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat thyroid tissue labeling Thyroid Peroxidase/TPO with ab275932 at 1/50 (8.98 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat thyroid is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)](https://www.abcam.com/ps/products/275/ab275935/Images/ab275935-1-anti-thyroid-peroxidasetpo-antibody-epr23574130-immunohistochemistry-frozen-mouse-thyroid.jpg)
Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)This data was developed using ab275932, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse thyroid tissue labeling Thyroid Peroxidase/TPO with ab275932 at 1/50 (8.98 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse thyroid is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)](https://www.abcam.com/ps/products/275/ab275935/Images/ab275935-3-benefits-of-recombinant-antibodies.png)
Anti-Thyroid Peroxidase/TPO antibody [EPR23574-130] - BSA and Azide free (ab275935)