Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16797-194] to JNK1 + JNK3 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis
Overview
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Product name
Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free
See all JNK1 + JNK3 primary antibodies -
Description
Rabbit monoclonal [EPR16797-194] to JNK1 + JNK3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251093 is the carrier-free version of ab191603. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251093 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16797-194 -
Isotype
IgG
Images
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All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : Full length human JNK1 recombinant protein
Lane 2 : Full length human JNK2 recombinant protein
Lane 3 : Full length human JNK3 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48,71 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab191603, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.
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All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab191603, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis data was developed using ab191603, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab191603, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lane 2 : Xenopus (X. tropicalis) muscle lysates
Lane 3 : MDCK (Canine kidney cell line) whole cell lysate
Lane 4 : MDBK (Bovine kidney cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab191603, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab191603, the same antibody clone in a different buffer formulation.
JNK1 + JNK3 were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab191603 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191603 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: K562 whole cell extract 10 µg (Input).
Lane 2: ab191603 IP in K562 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191603 in K562 whole cell extract.
Blocking and dilution buffer: 5% NFDM/TBST.
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