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Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16797-194] to JNK1 + JNK3 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis

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Overview

  • Product name

    Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free
    See all JNK1 + JNK3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16797-194] to JNK1 + JNK3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251093 is the carrier-free version of ab191603. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251093 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16797-194
  • Isotype

    IgG

Images

  • Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

    Lane 1 : Full length human JNK1 recombinant protein
    Lane 2 : Full length human JNK2 recombinant protein
    Lane 3 : Full length human JNK3 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48,71 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab191603, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.

  • Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab191603, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Rat kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab191603, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab191603, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

    Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
    Lane 2 : Xenopus (X. tropicalis) muscle lysates
    Lane 3 : MDCK (Canine kidney cell line) whole cell lysate
    Lane 4 : MDBK (Bovine kidney cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab191603, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)

    This data was developed using ab191603, the same antibody clone in a different buffer formulation.

    JNK1 + JNK3 were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab191603 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191603 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).

    Lane 2: ab191603 IP in K562 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191603 in K562 whole cell extract.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)
    Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free (ab251093)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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