Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)

Key features and details

  • Mouse monoclonal [9B6] to Giantin - Golgi Marker
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Giantin antibody [9B6] - Golgi Marker
    See all Giantin primary antibodies

  • Description

    Mouse monoclonal [9B6] to Giantin - Golgi Marker

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Full length protein corresponding to Rat Giantin aa 1 to the C-terminus.
    Database link: NP_620240.1

  • Positive control

    • ICC/IF: HeLa and HEK-293 cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    IgG fraction

  • Clonality

    Monoclonal

  • Clone number

    9B6

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
    Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)

    ICC/IF image of ab37266 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab37266, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
    Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266) This image is courtesy of an Abreview submitted by Dr Kirk McManus
    ab37266 (1/200) staining Giantin in assynchronous HeLa cells (green). Cells were fixed in Methanol and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to Abreview for further experimental details.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
    Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
    ICC/IF image of ab37266 stained Hek293 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37266, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)

© 2021 Astana Biomed Group LLP. All rights reserved.