Anti-TRPM2 antibody (ab11168)
Key features and details
- Rabbit polyclonal to TRPM2
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TRPM2 antibody
See all TRPM2 primary antibodies -
Description
Rabbit polyclonal to TRPM2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide, which represents a portion of the human transient receptor potential cation channel, subfamily M, member 2 encoded within exon 13 (LocusLink ID 7226).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRPM2 antibody (ab11168)All lanes : Anti-TRPM2 antibody (ab11168) at 0.1 µg
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) membrane preparation at 50 µg
Lane 2 : Jurkat membrane preparation at 15 µg
Lane 3 : Jurkat membrane preparation at 5 µg
Developed using the ECL technique.
Predicted band size: 171 kDa
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM2 antibody (ab11168)IHC image of ab11168 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunoprecipitation - Anti-TRPM2 antibody (ab11168)Detection of human TRPM2 by western blot of immunoprecipitates. Samples: Membrane Prep (0.5 or 1.0mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody A300-414A (lot A300-414A-3) used for IP at 6ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody A300-413A. For blotting immunoprecipitated TRPM2, A300-414A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
