Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human BMP2 protein (Fc Chimera) (ab214607)

Key features and details

  • Expression system: CHO cells
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human BMP2 protein (Fc Chimera)
    See all BMP2 proteins and peptides

  • Biological activity

    Shows the biological function of the BMP-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain in vivo. Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 2.0 -3.5μg/ml.

  • Purity

    >= 98 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    CHO cells

  • Accession

    P12643

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      RKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLN STNH AIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQ DMVVEGCGCR

    • Amino acids

      289 to 396

    • Additional sequence information

      The extracellular domain of Human BMP2 (aa 289-396) is fused to the N-terminus of the Fc region of a mutant Human IgG1 (NP_001191.1).

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab214607 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        Functional Studies

      • Form

        Lyophilized

      • Additional notes

        Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

        Constituent: 100% PBS

        0.2µm-filtered

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstitute in 50µl sterile water. Add 1X PBS to the desired protein concentration.

      General Info

      • Alternative names

        • BDA2
        • BMP-2
        • BMP-2A
        • Bmp2
        • BMP2_HUMAN
        • BMP2A
        • Bone morphogenetic protein 2
        • Bone morphogenetic protein 2A
        see all

      • Function

        Induces cartilage and bone formation.

      • Tissue specificity

        Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

      • Sequence similarities

        Belongs to the TGF-beta family.

      • Cellular localization

        Secreted.
      • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab214607? Please let us know so that we can cite the reference in this datasheet.

    ab214607 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • BDA2
      • BMP-2
      • BMP-2A
      • Bmp2
      • BMP2_HUMAN
      • BMP2A
      • Bone morphogenetic protein 2
      • Bone morphogenetic protein 2A
      see all

    • Function

      Induces cartilage and bone formation.

    • Tissue specificity

      Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

    • Sequence similarities

      Belongs to the TGF-beta family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Recombinant human BMP2 protein (Fc Chimera) (ab214607)

    Sign up