Prestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa) (ab234617)
Overview
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Product name
Prestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa)
See all Prestained Protein Ladder reagents -
Description
Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Prestained Protein Ladder ab234617 is a blue protein standard with 11 pre-stained proteins covering a range of MW from 10-180 kDa. ab234617 is designed for monitoring protein seperation in SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 10-180 kDa
- Ready to use: The protein ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.50
Constituents: 0.44% Tris phosphate, 2% Sodium lauryl sulfate, 0.003% DTT, 21.6% Urea, 15% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
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Images
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SDS-PAGE - Prestained Protein Ladder – Mid-range molecular weight (10 – 180 kDa) (ab234617)
SDS-PAGE analysis with ab234617.
Gel 1: Tris-Glycine 10%
Gel 2: Bis-Tris 4-12% MOPS buffer
Gel 3: Bis-Tris 4-12% MES buffer.
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.
