Prestained Protein Ladder - Mid-range molecular weight (10-175 kDa) (ab115832)
Overview
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Product name
Prestained Protein Ladder - Mid-range molecular weight (10-175 kDa)
See all Prestained Protein Ladder reagents -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Prestained Protein Ladder ab115832 contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. This protein ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
This product was previously called Prism Protein Ladder (10-175 kDa).
Key product features:
- Broad range: 10-175 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easy to identify: Includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye.
- Sharp bands
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 0.01% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 15% Glycerol -
Concentration information loading... -
Research areas
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Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
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Images
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SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10-175 kDa) (ab115832)SDS-PAGE with ab115832. Gel 1: Tris-Glycine 15%, Gel 2: Bis-Tris 4-12% MOPS buffer and Gel 3: Bis-Tris 4-12% MES Buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.
