Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa) (ab234592)
Overview
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Product name
Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa)
See all Prestained Protein Ladder reagents -
Description
Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) -
Tested applications
Suitable for: WB, SDS-PAGEmore details -
General notes
Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 6.5-270 kDa
- Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.50
Preservative: 0.01% Sodium azide
Constituents: 1.37% Tris phosphate, 0.03% EDTA, 2% Sodium lauryl sulfate, 0.15% DTT, 33% Glycerol -
Concentration information loading... -
Research areas
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Alternative names
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
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Images
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SDS-PAGE - Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa) (ab234592)
SDS-PAGE analysis with ab234592.
Gel 1: Tris-Glycine 4-20%
Gel 2: Bis-Tris 4-12% MES buffer.
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
