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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Anti-WDR1 antibody [EPR8793] (ab173574)

Price and availability

298 185 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-WDR1 antibody [EPR8793] (ab173574)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8793] to WDR1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-WDR1 antibody [EPR8793]
    See all WDR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8793] to WDR1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human WDR1 aa 300-400. The exact sequence is proprietary.
    Database link: O75083

  • Positive control

    • WB: Human heart tissue lysate. Jurkat, Raji, HT-1080 and U87-MG cell lysates. IHC: Human skeletal muscle tissue and endometrial carcinoma tissue. ICC/IF: Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8793
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly

Images

  • Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/200 dilution + Human heart tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 66 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Buffer concentration: 5% NFDM /TBST. 

    For human samples, please use lower antibody dilution or upload more of the protein.

  • Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
    All lanes : Anti-WDR1 antibody [EPR8793] (ab173574) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    Buffer concentration: 5% NFDM /TBST 

    We are unsure as to the identity of the extra band below 75kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574)

    Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).

  • Anti-WDR1 antibody [EPR8793] (ab173574)
    Anti-WDR1 antibody [EPR8793] (ab173574)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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