PLTP Activity Assay Kit II (Fluorometric) (ab196998)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Plasma, Purified protein, Serum
Overview
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Product name
PLTP Activity Assay Kit II (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Purified protein -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
PLTP Activity Assay Kit II (Fluorometric) (ab196998) uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Phospholipid Transfer Protein (PLTP). PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/Em = 465/535 nm). The fluorometric intensity is directly proportional to the amount of phospholipid transferred. The kit contains rabbit serum as positive control for assay validation.
This assay can measure PLTP activity in serum but can also be used for testing activity of recombinant PLTP protein.
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Notes
Phospholipid Transfer Protein (PLTP) is a protein found in mammalian plasma that plays an important role in transferring phospholipids between high-density lipoprotein (HDL) molecules and in the modulation of HDL size and composition, as well as in controlling plasma HDL levels. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability.
Due to its role in cholesterol transport, PLTP maybe promote atherosclerosis and is, therefore, considered a promising target for pharmacological intervention.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests Donor Molecule 1 x 0.2ml PLTP Acceptor Molecule 1 x 0.5ml PLTP Assay Buffer 1 x 20ml Positive Control 1 x 0.1ml -
Research areas
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Relevance
Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance. -
Cellular localization
Secreted. -
Alternative names
- HDLCQ9
- Lipid transfer protein II
- Phospholipid transfer protein
- PLTP
Images
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Functional Studies - PLTP Activity Assay Kit II (Fluorometric) (ab196998)
Donor Molecule Standard Curve
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Functional Studies - PLTP Activity Assay Kit II (Fluorometric) (ab196998)Measurement of PLTP activity of rabbit serum (1 µL), Human plasma (1 µL).