Anti-p53 antibody (ab17990)
Key features and details
- Rabbit polyclonal to p53
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-p53 antibody
See all p53 primary antibodies -
Description
Rabbit polyclonal to p53 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
-
Immunogen
Synthetic peptide corresponding to Human p53. Synthetic peptide (Human) - the immunogen lies within the region between residues 50 and 100 of human tumor protein p53.
Database link: P04637
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-p53 antibody (ab17990)
Western blot on 293T cells using ab17990 at 0.04
µ g/ml.
Lane 1: 293T cells mock treated with an siRNA for p53
Lane 2: 293T cells treated with an siRNA for p53
Lysates: 100µ g/lane.
Detection: Chemiluminescence with an exposure time of less than 1 minute.Western blot on 293T cells using ab17990 at 0.04
µg/ml.
Lane 1: 293T cells mock treated with an siRNA for p53
Lane 2: 293T cells treated with an siRNA for p53
Lysates: 100µg/lane.
Detection: Chemiluminescence with an exposure time of less than 1 minute. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody (ab17990)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling p53 with ab17990 at 1/1000 (1µg/ml). Detection: DAB. -
Flow Cytometry - Anti-p53 antibody (ab17990)ab17990 at 1.5ug/ml staining p53 in human Jurkat cell line by Flow Cytometery. Cells were fixed in 1.5% paraformaldehyde, and permeabilized in 90% methanol. A FITC-conjugated goat anti rabbit was used as secondary. The black line indicates control anti KLH IgG and the red line repersents ab17990 staining.
