Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
![Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](https://www.abcam.com/ps/products/191/ab191603/Images/ab191603-242392-191603WBa.jpg "Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16797-194] to JNK1 + JNK3
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis, Recombinant fragment
Overview
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Product name
Anti-JNK1 + JNK3 antibody [EPR16797-194]
See all JNK1 + JNK3 primary antibodies -
Description
Rabbit monoclonal [EPR16797-194] to JNK1 + JNK3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseRatCowDogHumanXenopus tropicalisRecombinant fragment
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Full length human JNK1 and JNK3 recombinant proteins; K562, HeLa, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, Neuro-2a, MDCK and MDBK whole cell lysates; Mouse brain, mouse heart, mouse kidney and rat kidney lysates; Xenopus (X. tropicalis) muscle lysates. IP: K562 whole cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16797-194 -
Isotype
IgG
Images
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Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : Full length human JNK1 recombinant protein
Lane 2 : Full length human JNK2 recombinant protein
Lane 3 : Full length human JNK3 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 48,71 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesRecombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](https://www.abcam.com/ps/products/191/ab191603/Images/ab191603-242391-191603WBb.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](https://www.abcam.com/ps/products/191/ab191603/Images/ab191603-242390-191603WBc.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](https://www.abcam.com/ps/products/191/ab191603/Images/ab191603-242389-191603WBd.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](https://www.abcam.com/ps/products/191/ab191603/Images/ab191603-242388-191603WBe.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lane 2 : Xenopus (X. tropicalis) muscle lysates
Lane 3 : MDCK (Canine kidney cell line) whole cell lysate
Lane 4 : MDBK (Bovine kidney cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](https://www.abcam.com/ps/products/191/ab191603/Images/ab191603-242387-191603IP.jpg)
Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)JNK1 + JNK3 were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab191603 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191603 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: K562 whole cell extract 10 µg (Input).
Lane 2: ab191603 IP in K562 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191603 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](https://www.abcam.com/ps/products/191/ab191603/Images/ab191603-7-benefits-of-recombinant-antibodies.png)
Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
