Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2605] to Tau (phospho S404) - BSA and Azide free
  • Suitable for: IHC-Fr, ICC, IP, Dot blot, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR2605] to Tau (phospho S404) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, ICC, IP, Dot blot, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Mouse primary cortical cultures;

  • General notes

    ab196364 is the carrier-free version of ab92676. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab196364 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)
    Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)

    Immunocytochemistry/ Immunofluorescence analysis of Embryonic mouse primary neural cells labeling Tau with purified ab92676 at 1:100 dilution (1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. 

    Confocal image showing positive staining in neuron.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92676).

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling Tau with Purified ab92676 at 1/50 (1.9 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92676).

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)
    Immunocytochemistry/ Immunofluorescence - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364) This image is courtesy of an anonymous Abreview.

    Mouse primary cortical cultures stained for Tau (phospho S404) (green) using ab92676 at 1/100 dilution in ICC/IF, followed by Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488®.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92676).

  • Dot Blot - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)
    Dot Blot - Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)

    Dot blot analysis of Tau (pS404) phosopho peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (phospho S404) with ab92676 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92676).

  • Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)
    Anti-Tau (phospho S404) antibody [EPR2605] - BSA and Azide free (ab196364)

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