Astana Biomed Group, an authorized Abcam distributor in Central Asia

PE Anti-6X His tag® antibody [EPR20547] (ab237338)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EPR20547] to 6X His tag®
  • Suitable for: ICC, Flow Cyt
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-6X His tag® antibody [EPR20547]
    See all 6X His tag® primary antibodies

  • Description

    PE Rabbit monoclonal [EPR20547] to 6X His tag®

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested applications

    Suitable for: ICC, Flow Cytmore details

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HEK-293T cells transfected with 6X His tag with GFP tag. Flow Cyt: HEK-293T cells transfected with 6X His tag with GFP tag.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20547

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - PE Anti-6X His tag® antibody [EPR20547] (ab237338)
    Immunocytochemistry - PE Anti-6X His tag® antibody [EPR20547] (ab237338)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells transfected with 6X His tag with GFP tag labelling 6X His tag with ab237338 at 1/100 (5 µg/ml). The nuclear counterstain was DAPI (Blue).

  • Flow Cytometry - PE Anti-6X His tag® antibody [EPR20547] (ab237338)
    Flow Cytometry - PE Anti-6X His tag® antibody [EPR20547] (ab237338)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T cells transfected with 6X His tag with GFP tag cells labelling 6X His tag with ab237338 at 1/500 dilution (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Left panel).

    Blocking with 10% Goat serum.

  • PE Anti-6X His tag® antibody [EPR20547] (ab237338)
    PE Anti-6X His tag® antibody [EPR20547] (ab237338)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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