PE Anti-6X His tag® antibody [EPR20547] (ab237338)
![PE Anti-6X His tag® antibody [EPR20547] (ab237338)](https://www.abcam.com/ps/products/237/ab237338/Images/ab237338-447547-Anti6X-His-tag-antibody-EPR20547-PE-immunocytochemistry-transfected-hek293t.jpg "PE Anti-6X His tag® antibody [EPR20547] (ab237338)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20547] to 6X His tag®
- Suitable for: ICC, Flow Cyt
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-6X His tag® antibody [EPR20547]
See all 6X His tag® primary antibodies -
Description
PE Rabbit monoclonal [EPR20547] to 6X His tag® -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: HEK-293T cells transfected with 6X His tag with GFP tag. Flow Cyt: HEK-293T cells transfected with 6X His tag with GFP tag.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20547 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - PE Anti-6X His tag® antibody [EPR20547] (ab237338)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells transfected with 6X His tag with GFP tag labelling 6X His tag with ab237338 at 1/100 (5 µg/ml). The nuclear counterstain was DAPI (Blue).
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![Flow Cytometry - PE Anti-6X His tag® antibody [EPR20547] (ab237338)](https://www.abcam.com/ps/products/237/ab237338/Images/ab237338-447546-Anti6X-His-tag-antibody-EPR20547-PE-flow-cytometry-transfected-hek293t.jpg)
Flow Cytometry - PE Anti-6X His tag® antibody [EPR20547] (ab237338)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T cells transfected with 6X His tag with GFP tag cells labelling 6X His tag with ab237338 at 1/500 dilution (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (ab209478) (Left panel).
Blocking with 10% Goat serum.
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![PE Anti-6X His tag® antibody [EPR20547] (ab237338)](https://www.abcam.com/ps/products/237/ab237338/Images/ab237338-456224-benefitsofrecombinantantibodies.jpg)
PE Anti-6X His tag® antibody [EPR20547] (ab237338)
