Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y179] to p53 (phospho S6) - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free
    See all p53 primary antibodies

  • Description

    Rabbit monoclonal [Y179] to p53 (phospho S6) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects p53 phosphorylation on Serine 6. It is predicted to react with p53 beta and gamma isoform, based on sequence homology. 

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa treated with etoposide whole cell lysate.

  • General notes

    ab247235 is the carrier-free version of ab32132 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247235 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)

    This data was developed using ab32132, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using ab32132 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Western blot - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    All lanes : Anti-p53 (phospho S6) antibody [Y179] (ab32132) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate - untreated
    Lane 2 : HepG2 whole cell lysate - treated with Etopside

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab32132, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Immunoprecipitation - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)

    This data was developed using ab32132, the same antibody clone in a different buffer formulation.
    Purified ab32132 at 1/50 dilution (2µg) immunoprecipitating p53 in HeLa treated with etoposide whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with etoposide whole cell lysate 10µg
    Lane 2 (+): ab32132 + HeLa treated with etoposide whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32132 in HeLa treated with etoposide whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)

    This data was developed using ab32132, the same antibody clone in a different buffer formulation.

    ab32132 showing positive staining in Lung adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    This data was developed using ab32132, the same antibody clone in a different buffer formulation.ab32132 showing positive staining in Colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Western blot - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    All lanes : Anti-p53 (phospho S6) antibody [Y179] (ab32132) at 1/5000 dilution

    Lane 1 : MCF7 cell lysate, non-treated
    Lane 2 : MCF7 cell lysate, treated with Actinomycin D for 3 hours
    Lane 3 : MCF7 cell lysate, treated with Actinomycin D for 6 hours
    Lane 4 : MCF7 cell lysate, treated with Actinomycin D for 18 hours

    Predicted band size: 44 kDa
    Observed band size: 53 kDa why is the actual band size different from the predicted?



    This data was developed using ab32132, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    This data was developed using ab32132, the same antibody clone in a different buffer formulation.ab32132 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)
    Anti-p53 (phospho S6) antibody [Y179] - BSA and Azide free (ab247235)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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