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Mouse TGF beta 1 ELISA Kit (ab119557)

Key features and details

  • Sensitivity: 7.8 pg/ml
  • Range: 31.3 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse TGF beta 1 ELISA Kit
    See all TGF beta 1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4 7.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4 5.8%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    7.8 pg/ml

  • Range

    31.3 pg/ml - 2000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124% - 141%
    Serum 92% - 103%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Abcam’s TGF beta 1 Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse TGF beta 1 concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate).

    Mouse TGF beta 1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Mouse TGF beta 1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mouse TGF beta 1 captured on the plate

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 2 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 6 units
    Biotinylated mouse anti-TGF beta 1 monoclonal antibody 1 x 120µl
    Microplate coated with monoclonal antibody to mouse TGF beta 1 (12 x 8 wells) 1 unit
    Mouse TGF beta 1 Standard lyophilized 2 vials
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml

  • Research areas

  • Function

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

  • Tissue specificity

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

  • Involvement in disease

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Post-translational
    modifications

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Alternative names

    • Cartilage-inducing factor
    • CED
    • Differentiation inhibiting factor
    • DPD1
    • LAP
    • Latency-associated peptide
    • Prepro transforming growth factor beta 1
    • TGF beta
    • TGF beta 1
    • TGF beta 1 protein
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta-5
    • TGF-beta1
    • TGFB
    • Tgfb-1
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • TGFbeta1
    • Transforming Growth Factor b1
    • Transforming Growth Factor beta 1
    • Transforming growth factor beta 1a
    • transforming growth factor beta-1
    • transforming growth factor, beta 1
    • Transforming Growth Factor-ß1
    see all

  • Database links

    Images

    • Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)
      Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)
      Standard curve of Ms TGFb, with background signal subtracted (duplicates; +/- SD).
    • Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)
      Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (ab119557)
      Mouse TGFb measured in activated biological fluids, background signal subtracted (duplicates +/- SD). * Samples without activation.
    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab119557.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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