Anti-AMH antibody - C-terminal (ab229212)
Key features and details
- Rabbit polyclonal to AMH - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AMH antibody - C-terminal
See all AMH primary antibodies -
Description
Rabbit polyclonal to AMH - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB Human
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Immunogen
Recombinant fragment within Human AMH (C terminal). The exact sequence is proprietary.
Database link: P03971 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Mouse ovary and testis tissues. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMH antibody - C-terminal (ab229212)All lanes : Anti-AMH antibody - C-terminal (ab229212) at 1/2500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 59 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (ab229212)Paraffin-embedded mouse ovary tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody - C-terminal (ab229212)Paraffin-embedded mouse testis tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-AMH antibody - C-terminal (ab229212)Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for AMH (green) using ab229212 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
