Mouse Leptin ELISA Kit (ab199082)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 33.6 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Leptin ELISA Kit
See all Leptin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 5.9% Inter-assay Sample n Mean SD CV% Serum 3 11.5% -
Sample type
Cell culture supernatant, Urine, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
33.6 pg/ml -
Range
93.75 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 80 77% - 82% Serum 111 110% - 114% Cell culture media 108 107% - 108% Hep Plasma 106 94% - 116% Cit plasma 104 100% - 111% serum free media 104 103% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse Leptin ELISA Kit (ab199082) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Leptin protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Leptin with 33.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause obesity. Circulating levels of Leptin are regulated by food intake, insulin levels and pregnancy status. Mouse leptin has 96% and 85% protein sequence identity to rat and human leptin, respectively.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Leptin Capture Antibody 1 x 600µl 10X Mouse Leptin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse Leptin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FLJ94114
- LEP
- LEP_HUMAN
see all -
Database links
- Entrez Gene: 16846 Mouse
- SwissProt: P41160 Mouse
- Unigene: 277072 Mouse
Images
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Other - Mouse Leptin ELISA Kit (ab199082)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Leptin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of Leptin in mouse serum and plasma (citrate).The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 4514.3 pg/mL in mouse serum and 2698.1 pg/mL in mouse plasma (Citrate).
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Interpolated concentrations of Leptin in rat serum, plasma (citrate), and plasma (heparin).The concentrations of Leptin were measured in duplicate and interpolated from the Leptin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 2983 pg/mL in rat serum, 1626 pg/mL in rat plasma (citrate) and 2134 pg/mL in rat plasma (heparin).
