Mouse Leptin ELISA Kit (ab108881)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.375 ng/ml - 24 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Leptin ELISA Kit
See all Leptin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.5% Inter-assay Sample n Mean SD CV% Overall 9.1% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.3 ng/ml -
Range
0.375 ng/ml - 24 ng/ml -
Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Leptin mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of leptin levels in plasma, serum and cell culture supernatants.
A Leptin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Leptin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leptin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 70X Biotinylated Mouse Leptin Antibody 1 x 90µl Chromogen Substrate 1 x 7ml Leptin Microplate (12 x 8 well strips) 1 unit Leptin Standard 1 x 2 vials Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FLJ94114
- LEP
- LEP_HUMAN
see all -
Database links
- Entrez Gene: 16846 Mouse
- SwissProt: P41160 Mouse
- Unigene: 277072 Mouse
Images
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Typical Standard Curve
Representative Standard Curve using ab108881
